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rankl  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology rankl
    Rankl, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 269 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+rankl/RANKL+Antibody/pm40783844-91-29-36
    Average 94 stars, based on 269 article reviews
    rankl - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Western Blot:

    Article Title: Role of DCAF8 in Mammary Ductal Elongation and Branching Morphogenesis.
    Article Snippet: .. For Western blotting, the following antibodies were used: anti-DCAF8 (#ab176809, abcam; 1:4000 dilution), anti-GAPDH (#5174, 1:10000 dilution), antiERβ (#sc-53494, Santa Cruz; 1:1000 dilution), anti-RANKL (#sc-52950, Santa Cruz, 1:1000 dilution), anti--actin (#A1978, Sigma-Aldrich, 1:8000 dilution), anti-ERα (#8644S, Cell Signaling Technology, 1:1000 dilution), anti-PR (#8757S, Cell Signaling Technology, 1:1000 dilution). .. For Imunochemistry and/or Immunofluorescence staining, the following antibodies were used: anti-DCAF8 (Sigma, #HPA027218), anti-ER (ThermoFisher, #MA5–24807), anti-ERα (Santa Cruz, #sc-8005), anti-PR (Cell Signaling Technology, #8757S).

    Membrane:

    Article Title: TMCO1-Deficient Mice Exhibit a High Incidence of Otitis Media Associated with Impaired Bone Homeostasis in the Middle Ear.
    Article Snippet: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 ajp.amjpathol.org 63 64 65 66 67 68 69 70 71 72 73 74 TMCO1-Deficient Mice Exhibit a High Incidence 75 76 of Otitis Media Associated with Impaired Bone 77 78 79 80 81 82 Homeostasis in the Middle Ear Yaning Dong,*y Peng Ma,z Shuli Wang,* Lan Wang,* Yingying Chen,x Fangfang Zhao,* Keyan Yang,x Xiaolin Zhang,x Hongchun Zhao,x Bo Li,* Ruishuang Geng,* Tie-shan Tang,{ Qingyin Zheng,*x and Tihua Zheng*

    Incubation:

    Article Title: TMCO1-Deficient Mice Exhibit a High Incidence of Otitis Media Associated with Impaired Bone Homeostasis in the Middle Ear.
    Article Snippet: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 ajp.amjpathol.org 63 64 65 66 67 68 69 70 71 72 73 74 TMCO1-Deficient Mice Exhibit a High Incidence 75 76 of Otitis Media Associated with Impaired Bone 77 78 79 80 81 82 Homeostasis in the Middle Ear Yaning Dong,*y Peng Ma,z Shuli Wang,* Lan Wang,* Yingying Chen,x Fangfang Zhao,* Keyan Yang,x Xiaolin Zhang,x Hongchun Zhao,x Bo Li,* Ruishuang Geng,* Tie-shan Tang,{ Qingyin Zheng,*x and Tihua Zheng*

    Article Title: Early cellular events of osteomucosal healing in the tooth extraction socket
    Article Snippet: .. After tissue blocking, the tissue sections were incubated overnight at 4°C with primary antibodies for anti-Rankl (C-20, 1:100; Santa Cruz Biotechnology, Inc. Santa Cruz, CA) or anti-OPG (ab183910, 1:100; Abcam, Cambridge, MA). .. The primary antibodies were detected by incubation with a corresponding biotinylated secondary antibody, an anti-goat IgG or anti-rabbit IgG secondary antibody conjugated with horseradish peroxidase (1:500; Jackson Immuno Research Laboratories, Inc., PA) for 30 minutes at 37°C, and then visualized with 3,3’Diaminobenzidine (DAB) solution (Abcam).

    Article Title: OPG-Producing B Cells and RANKL-Expressing T Cells Define Immune Signatures Predictive of Bone Metastases in Breast Cancer
    Article Snippet: Endogenous peroxidase activity was blocked using Leica Peroxidase Block (Leica Biosystems, RRID:SCR_016559) for 5 minutes, followed by nonspecific binding blockade using Leica Protein Block (Leica Biosystems, RRID:SCR_016559) for 5 minutes at room temperature. .. Sections were incubated with the following primary antibodies: anti-CD4 (clone SP35, Cell Marque, RRID:AB_2877883), anti-CD3 (clone MRQ-39, Cell Marque, RRID:AB_2880051), anti-CD20 (clone L26, Cell Marque, RRID:AB_2877881), anti-OPG (clone E-10, Santa Cruz Biotechnology, RRID:AB_627683), and anti-RANKL (clone G-1, Santa Cruz Biotechnology, RRID:AB_2189774) overnight at 4°C. .. After TBS washing, the sections were incubated for 30 minutes with a secondary biotinylated antibody (Leica Post Primary, Leica Biosystems, RRID:SCR_016559), followed by a 30-minute incubation with the Novolink Polymer Detection System (Leica Biosystems, RRID:SCR_016559).

    Tissue:

    Article Title: Early cellular events of osteomucosal healing in the tooth extraction socket
    Article Snippet: .. After tissue blocking, the tissue sections were incubated overnight at 4°C with primary antibodies for anti-Rankl (C-20, 1:100; Santa Cruz Biotechnology, Inc. Santa Cruz, CA) or anti-OPG (ab183910, 1:100; Abcam, Cambridge, MA). .. The primary antibodies were detected by incubation with a corresponding biotinylated secondary antibody, an anti-goat IgG or anti-rabbit IgG secondary antibody conjugated with horseradish peroxidase (1:500; Jackson Immuno Research Laboratories, Inc., PA) for 30 minutes at 37°C, and then visualized with 3,3’Diaminobenzidine (DAB) solution (Abcam).

    Blocking Assay:

    Article Title: Early cellular events of osteomucosal healing in the tooth extraction socket
    Article Snippet: .. After tissue blocking, the tissue sections were incubated overnight at 4°C with primary antibodies for anti-Rankl (C-20, 1:100; Santa Cruz Biotechnology, Inc. Santa Cruz, CA) or anti-OPG (ab183910, 1:100; Abcam, Cambridge, MA). .. The primary antibodies were detected by incubation with a corresponding biotinylated secondary antibody, an anti-goat IgG or anti-rabbit IgG secondary antibody conjugated with horseradish peroxidase (1:500; Jackson Immuno Research Laboratories, Inc., PA) for 30 minutes at 37°C, and then visualized with 3,3’Diaminobenzidine (DAB) solution (Abcam).

    other:

    Article Title: Upregulated bone morphogenetic protein 8A (BMP8A) in triple negative breast cancer (TNBC) and its involvement in the bone metastasis
    Article Snippet: Anti-RANKL , Rabbit , 35.5, 27.7, 30.5 , Santa Cruz , sc9073 , 1:1,000.



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    CIHH downregulates <t>RANKL</t> expression and increases <t>the</t> <t>OPG/RANKL</t> ratio in delayed replanted teeth. A Immunohistochemistry staining showing OPG expression in the root and periapical tissue of NN and CIHH groups of delayed replanted teeth. Scale bar: 100 μm. B Immunohistochemistry staining showing RANKL expression in the root and periapical tissue of the NN and CIHH groups. Scale bar: 100 μm. C - D Representative Western blot bands ( C ) and quantitative analysis ( D ) showing OPG and RANKL protein expressions in the root and periapical tissue of NN and CIHH groups of delayed replanted teeth. D dentin; P: periodontal ligament; B: bone
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    CIHH downregulates <t>RANKL</t> expression and increases <t>the</t> <t>OPG/RANKL</t> ratio in delayed replanted teeth. A Immunohistochemistry staining showing OPG expression in the root and periapical tissue of NN and CIHH groups of delayed replanted teeth. Scale bar: 100 μm. B Immunohistochemistry staining showing RANKL expression in the root and periapical tissue of the NN and CIHH groups. Scale bar: 100 μm. C - D Representative Western blot bands ( C ) and quantitative analysis ( D ) showing OPG and RANKL protein expressions in the root and periapical tissue of NN and CIHH groups of delayed replanted teeth. D dentin; P: periodontal ligament; B: bone
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    Image Search Results


    CIHH downregulates RANKL expression and increases the OPG/RANKL ratio in delayed replanted teeth. A Immunohistochemistry staining showing OPG expression in the root and periapical tissue of NN and CIHH groups of delayed replanted teeth. Scale bar: 100 μm. B Immunohistochemistry staining showing RANKL expression in the root and periapical tissue of the NN and CIHH groups. Scale bar: 100 μm. C - D Representative Western blot bands ( C ) and quantitative analysis ( D ) showing OPG and RANKL protein expressions in the root and periapical tissue of NN and CIHH groups of delayed replanted teeth. D dentin; P: periodontal ligament; B: bone

    Journal: BMC Oral Health

    Article Title: Chronic intermittent hypobaric hypoxia attenuates root resorption following delayed tooth replantation by upregulating OPG/RANKL signaling pathway

    doi: 10.1186/s12903-026-08135-7

    Figure Lengend Snippet: CIHH downregulates RANKL expression and increases the OPG/RANKL ratio in delayed replanted teeth. A Immunohistochemistry staining showing OPG expression in the root and periapical tissue of NN and CIHH groups of delayed replanted teeth. Scale bar: 100 μm. B Immunohistochemistry staining showing RANKL expression in the root and periapical tissue of the NN and CIHH groups. Scale bar: 100 μm. C - D Representative Western blot bands ( C ) and quantitative analysis ( D ) showing OPG and RANKL protein expressions in the root and periapical tissue of NN and CIHH groups of delayed replanted teeth. D dentin; P: periodontal ligament; B: bone

    Article Snippet: Membranes were blocked with 5% skim milk and incubated with a mouse anti-OPG primary antibody (1:1000, Huabio, China) or a rabbit anti-RANKL primary antibody (1:1000, Huabio, China) overnight at 4 °C.

    Techniques: Expressing, Immunohistochemistry, Staining, Western Blot